RNA isolation and reverse transcription protocol: Cells in culture By Dr. Richard Pattern, Tufts-New England Medical Center, Boston. Procedure for the isolation, DNase treatment and reverse transcription of RNA from cell culture. RNA isolation procedure for cells 1.
Isolation and analysis of total RNA from chick embryos, cDNA synthesis and PCR analysis. Objective: to become familiar with molecular techniques used to analyze gene expression. Procedure: We will isolate total RNA (a mix of ribosomal RNA, transfer RNA and mRNA) by disrupting the cells of the.